Table 2 Generation of GGTA1-edited rabbits via the CRISPR/Cas9 system
From: α-Gal antigen-deficient rabbits with GGTA1 gene disruption via CRISPR/Cas9
Recipients | gRNA/Cas9 mRNA (ng/μL) | Embryos transferred | Pregnancy | Pups obtained (% transferred) | Pups with mutations (%) |
|---|---|---|---|---|---|
1 | 40/200 | 60 | Yes | 4 (6.7%) | 4 (100%) |
2 | 40/200 | 54 | Yes | 5 (9.3%) | 5 (100%) |
3 | 40/200 | 52 | No | 0 (0%) | 0 (0%) |
4 | 40/200 | 58 | Yes | 4 (6.9%) | 3 (75.0%) |
Total | / | 224 | / | 13 (5.8%) | 12 (92.3%) |