Table 1 Oligos synthesized for GGTA1 sgRNAs
From: α-Gal antigen-deficient rabbits with GGTA1 gene disruption via CRISPR/Cas9
Target gene | Target site | PAM | Oligonucleotide1 | Oligonucleotide2 |
|---|---|---|---|---|
GGTA1-sgRNA1 | CTCTCATAGGTAAATTCGTC | AGG | TAGGCTCTCATAGGTAAATTCGTC | AAACGACGAATTTACCTATGAGAG |
GGTA1-sgRNA2 | TTTTGGAGGAACACCCCTTC | AGG | TAGGTTTTGGAGGAACACCCCTTC | AAACGAAGGGGTGTTCCTCCAAAA |