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Fig. 5 | BMC Genomic Data

Fig. 5

From: A detailed workflow to develop QIIME2-formatted reference databases for taxonomic analysis of DNA metabarcoding data

Fig. 5

Comparison of classification accuracy at the species level from ITS2 and rbcL databases developed in this work and from previous studies. The comparison of classification accuracy is presented for ITS2 (A) and rbcL (B) databases. The pseudo-realistic classification accuracy (i.e. when subsets of reference sequences are blasted against the remaining ones and may thus not have an exact match in the training database) has been computed using a k-fold CV approach. The best possible classification accuracy (i.e. when all reference sequences are blasted against themselves and have thus an exact match in the training database) has been calculated using a leaked CV approach

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