Fig. 1From: A detailed workflow to develop QIIME2-formatted reference databases for taxonomic analysis of DNA metabarcoding dataFlowchart representing the major steps of DB4Q2 to develop reference databases. Sequences can be directly downloaded from the NCBI website or extracted offline from the local nt BLAST database after having downloaded the list of sequence accession numbers. *Optional steps, the choice is left to the user whether or not to include them in the workflowBack to article page