Skip to main content

Table 1 PAM motifs according to CRISPR-Cas type but regardless of the contained phages

From: Bio-informatic analysis of CRISPR protospacer adjacent motifs (PAMs) in T4 genome

CRISPR-Cas
system types
From Organism PAM Sequence
(5' to 3')
Reference
II-A
I-C
IV
Streptococcus pyogenes
S. agalactiae
Listeria monocytogenes
Francisella novicida (FnCas9)
NGG
NAG
(A/T)GG
[7, 16, 17]
NGG [18]
II-A Staphylococcus aureus NGRRT
NGRRN
NNGRRT
TTGGGT
[19,20,21]
II- C Neisseria meningitidis NNNNGATT [16]
II-A Streptococcus thermophilus NNAGAAW
NGGNG
[16, 17, 22, 23]
V-A
II-A
Lachnospiraceae bacterium TTTV
TCTA
[24]
V-A Cas12a
(AsCas12a, AsCpf1)
Acidaminococcus sp. GTTV
GCTV
TATA (eng. Cpf1)
TTTV
[10, 25]
I-E Escherichia coli C(T/A)T [17, 26, 27]
I-E Escherichia coli 5’-ATG-3’
5’-AAG-3’
Its complement
5′-CTT-3′
[28, 29]
I-E Escherichia coli AWG [30]
I-A S. solfataricus TCN
CCN
[31,32,33]