Fig. 6

Transcription regulator networks (TRNs) of activity of regulators and DEGs. TRNs diagram of regulator activities and DEGs based on ARACNe and VIPER algorithm. TRNs diagram illustrated transcriptional factors (TFs) and potential target genes (TGs). VIPER was conducted to identify activity of TFs and TGs, then inferred potential master regulators (MRs) and mechanisms by regulating of EMP2 in different conditions. Cytoscape software (version: 3.8.0) was used for visualizing TRNs. In this study, we defined “node” as either a TF or a TG, “edge” as a TF-TF, TF-TG or TG-TG relationship. “MR” was defined as a TF or a TG having many connections with others, which were marked with red color in the diagram. (p < 0.01, FDR q < 0.05, overlap cutoff > 0). A TRN of comparisons of EMP2-OE hRPECs vs VC under hypoxic condition, B TRN of comparisons of EMP2-KD vs WT under hypoxic condition. TRNs, transcription regulatory networks, MRs, master regulators