Fig. 2

Identification of differential expressed genes (DEGs) in EMP2-regulated RPE cells. Differentially Expressed Genes (DEGs) Enrichment Venn Diagram and its bar graph. Venn diagrams were created using the web-based tool http://bioinformatics.psb.ugent.be/webtools/Venn/. Identifying DEGs and up- and down- regulated DEGs terms in deferentially expressed sequences and regulated situation in treated groups vs control groups. DESeq2 R package was used in different expression gene counting. Cutoff value was absolute log2FC > 1 and P value < 0.05. A DEGs in four comparisons under hypoxic condition, B DEGs in four comparisons under normoxic condition, C-D Up- and down- regulated DEGs in comparisons under hypoxic and normoxic condition, respectively. Colors display the expression levels of each DEGs, as pink and purple indicate down- and up-regulated genes in treated group comparing with control group, respectively. DEGs: differentially expressed genes, kd_vc: EMP2-knocked down hRPECs vs vector control hRPECs, kd_wt: EMP2-knocked-down hRPECs vs wild type hRPECs, oe_vc: EMP2-over expressed hRPECs vs vector control hRPECs, oe_wt: EMP2-over expressed hRPECs vs wild type hRPECs