Fig. 3

Structural analysis of predicted and known Nsp5 cleavage motifs. a NetCorona scores are shown for all P5-P4’ motifs surrounding glutamine residues in three datasets of human proteins, binned by score differences of 0.01. The distributions of scores were not statistically different from one another. b Despite a high NetCorona score in ACHE, the motif’s location in the core of the protein leads to a low Nsp5 access score. c TAB1 contains several motifs predicted to be cleaved, including at Q108 and Q132. The Nsp5 access score is slightly higher for the Q132 motif due to the greater accessible surface area (ASA). d DHX15 contains the motif with the highest Nsp5 access score observed in the human proteins studied, located on the C-terminus of the protein. e SARS-CoV-2 proteins Nsp15 and Nsp16 contain the native Nsp5 cleavage motif with the lowest Nsp5 access score calculated (487), which helped provide a cut-off to Nsp5 access scores in human proteins. f The Nsp5 access score of human protein motifs are indicated, binned by score differences of 50. 92 motifs in 92 unique human proteins have a Nsp5 access score > 500