Table 2 qPCR primer sequences of target genes used for RNA-Seq validation

Gene Name

References

Forward primer (5′-3′)

Reverse primer (5′-3′)

Apolipoprotein E (APOE)

CTGCGTTGCTGGTCACATTCC

CGCTCTGCCACTCGGTCTG

[83]

WNT1 inducible signaling pathway protein 1 (WISP1)

GAAGCAGTCAGCCCTTATG

CTTGGGTGTAGTCCAGAAC

[84]

Rho GTPase activating protein 6 (ARHGAP6)

GGGAGGGAGGCATTCATCTAC

GTGGCCCACCAGCATAAAC

[85]

growth differentiation factor 15 (GDF15)

CCAAAGACTGCCACTGCATA

GAATCGGGTGTCTCAGGAAC

[86]

retinol binding protein 4 (RBP4)

TACTCCTTCGTGTTTTCCCGG

TAACCGTTGTGGACGATCAGC

[87]

Gene Name

References

prostaglandin E receptor 2 (PTGER2)

AGGAGACGGACCACCTCATTC

GCCTAAGGATGGCAAAGACCC

[88]

snail family transcriptional repressor 1 (SNAI1)

GGTTCTTCTGCGCTACTGCT

TAGGGCTGCenunTGGAAGGTAAA

[89]

Gene Name

References

insulin like growth factor binding protein 2 (IGFBP2)

GCCCTCTGGAGCACCTCTACT

CATCTTGCACTGTTTGAGGTTGTAC

[90]

ras related dexamethasone induced 1 (RASD1)

CCACCGCAAGTTCTACTCCAT

CCAGGATGAAAACGTCTCCTGT

[91]

fatty acid binding protein 4 (FABP4)

GCCAGGAATTTGACGAAGTCAC

TTCTGCACATGTACCAGGACAC

[92]

prostaglandin I2 synthase (PTGIS)

CTGGTTGGGGTATGCCTTGG

TCATCACTGGGGCTGTAATGT

[93]

apolipoprotein L3 (APOL3)

GCAAGGGACATGATGCCAGA

AAGAGTTTCCCCAAGTCAAGAGG

[94]

phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (PIK3C2B)

CAGGCTTCAAGAGGCACTCA

TGGTCATCATTCACCGTCCG

[95]

GAPDH

AGGGCTGCTTTTAACTCTGGT

CCCCACTTGATTTTGGAGGGA

[17]