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Fig. 6 | BMC Genomic Data

Fig. 6

From: Integrated analysis of DNA methylome and transcriptome reveals the differences in biological characteristics of porcine mesenchymal stem cells

Fig. 6

RNA-seq and MeDIP-seq data validation by RT-qPCR and Sequenom MassARRAY, respectively. The expression and promoter methylation levels of three representative genes (HOXB5, FGF21, and CYP26A1) were validated by RT-qPCR and Sequenom MassARRAY, respectively. A HOXB5, B FGF21, and C CYP26A1. The expression levels of these three genes in BMMSCs and UCMSCs are shown in the left panel. Error bars denote standard errors of means (* represents P < 0.05, *** represents P < 0.001). The right panel shows the Sequenom MassARRAY results. Each dot corresponds to one CpG position in the genome sequence. The colored bar summarizes the methylation level at that position, with blue indicating methylation (100%) and yellow indicating a lack of methylation (0%). Both analyses were performed with three biological replicates for each MSC. Results of the validation of other DEGs or differentially methylated promoter regions are shown in Additional file: Table S3

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