Table 8 Characteristics of the sheep microsatellite markers
From: Morphological and microsatellite DNA diversity of Djallonké sheep in Guinea-Bissau
Microsatellite | Primers | Sequences of primers | Nucleotide pattern | Number of chromosome | Hybridization temperature (°C) | Multiplex | Theoretical size |
|---|---|---|---|---|---|---|---|
OarJMP58 | Forward | GAAGTCATTGAGGGGTCGCTAACC | Di | OAR 26 | 58 | 1 | 145–169 |
Reverse | CTTCATGTTCACAGGACTTTCTCTG | ||||||
MAF214 | Forward | GGGTGATCTTAGGGAGGTTTTTGGAGG | Di | OAR 16 | 58 | 2 | 174–282 |
Reverse | AATGCAGGAGATCTGAGGCAGGGACG | ||||||
ILSTS5 | Forward | GGAAGCAATGAAATCTATAGCC | Di | OAR 7 | 55 | 3 | 174–218 |
Reverse | TGTTCTGTGAGTTTGTAAGC | ||||||
MAF65 | Forward | AAAGGCCAGAGTATGCAATTAGGAG | Di | OAR15 | 60 | 2 | 123–127 |
Reverse | CCACTCCTCCTGAGAATATAACATG | ||||||
OarFCB193 | Forward | TTCATCTCAGACTGGGATTCAGAAAGGC | Di | OAR 11 | 54 | 3 | 174–218 |
Reverse | GCTTGGAAATAACCCTCCTGCATCCC | ||||||
OarFCB304 | Forward | CCCTAGGAGCTTTCAATAAAGAATCGG | Di | OAR 19 | 56 | 3 | 150–188 |
Reverse | CGCTGCTGTCAACTGGGTCAGGG | ||||||
ILSTS11 | Forward | GCTTGCTACATGGAAAGTGC | Di | OAR 9 | 55 | 1 | 256–294 |
Reverse | CTAAAATGCAGAGCCCTACC | ||||||
MCM140 | Forward | GTTCGTACTTCTGGGTACTGGTCTC | Di | OAR 6 | 60 | 1 | 167–193 |
Reverse | GTCCATGGATTTGCAGAGTCAG | ||||||
SRCRSP1 | Forward | TGCAAGAAGTTTTTCCAGAGC | Di | OAR 13 | 54 | 1 | 116–148 |
Reverse | ACCCTGGTTTCACAAAAGG | ||||||
OarCP34 | Forward | GCTGAACAATGTGATATGTTCAGG | Di | OAR 3 | 50 | 4 | 112–130 |
Reverse | GGGACAATACTGTCTTAGATGCTGC | ||||||
OarCB226 | Forward | CTATATGTTGCCTTTCCCTTCCTGC | Di | OAR 2 | 60 | 3 | 119–153 |
Reverse | GTGAGTCCCATAGAGCATAAGCTC | ||||||
MAF70 | Forward | CACGGAGTCACAAAGAGTCAGACC | Di | OAR 4 | 60 | 4 | 124–166 |
Reverse | GCAGGACTCTACGGGGCCTTTGC |