Fig. 1
From: The mtDNA mutation spectrum in the PolG mutator mouse reveals germline and somatic selection
Methods to quantify abundance of mtDNA mutations. A. The pipeline used to call and classify mtDNA mutations B. The reference (Ref) shows a hypothetical 4 base pair mtDNA genome with 2 reads (Rd 1 and Rd. 2) aligned. Example mutation types, counts, and frequencies are displayed for two reads each from liver and brain tissue. Red bases highlight mutations. Mutations that occur in both tissues are germline, and mutations that occur in only one are somatic. Count defines numbers of unique variants, while frequency defines prevalence of a particular variant