Table 2 A summary of the broomcorn millet BESs and the anchoring results of the broomcorn millet BAC clones to the longmi 4 genome using the BESs
Categories | Short BESs | Long BESs | Integrity |
|---|---|---|---|
BAC end sequences | |||
Clones in superpool | 9216 | 9216 | |
Clones with successful BESs | 9126 | 7725 | |
Clones with paired BESs | 7790 | 3890 | |
Clones with single-end BESs | 1336 | 3835 | |
Clones with only forward BES | 921 | 2189 | |
Clones with only reverse BES | 415 | 1646 | |
Total successful BESs | 17,811 (96.63%) | 12,721 (66.17%) | |
Alignment | |||
Aligned BESs with repeats unmasked | 14,862 (83.44%) | 12,120 (95.28%) | |
Single-hit BESs | 12,971 | 12,120 | |
Multi-hit BESs | 1891 | 0 | |
Aligned BESs with repeats masked | 7760 (43.57%) | 10,626 (83.53%) | |
Single-hit BESs | 7295 | 10,626 | |
Multi-hit BESs | 465 | 0 | |
Anchoring to reference sequences | |||
Clones anchored to single sites | 5795 | 6973 | 8262 |
Clones anchored with single BES | 2507 | 3907 | 2871 |
Clones anchored with paired BESs | 3288 | 3066 | 5391 |