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Fig. 2 | BMC Genomic Data

Fig. 2

From: Genomic resources of broomcorn millet: demonstration and application of a high-throughput BAC mapping pipeline

Fig. 2

The strategy of BAC end sequence localization based on CAPSS. Twenty-four 384-plates are arranged to a square superpool, and then 96 row pools and 96 column pools are prepared and sequenced by NGS platform. The sequences of each pool are assembled into contigs. If a contig (especially, BAC end sequences, abbr. BES) is shared in a row pool and a column pool, it will be assigned to the well at the intersection of the row pool and the column pool. BAC clones will be further mapped to the reference genome according to assigned contigs

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