Fig. 2

The expression and sequence similarity of hsa-mir-548 family members in reproductive tissue samples. (A) The average number of hsa-mir-548 family members present in reproductive tissues with cut-off > 10 counts per million (CPM). (B) The expression levels of hsa-miR-548ba in reproductive tissues. The sequencing results are displayed as a mean of CPM ± SEM. CGC–cumulus granulosa cells (n = 3), MGC–mural granulosa cells (n = 3), testis (n = 5), SF–seminal fluid (n = 1), pre-receptive endometrium (n = 12), receptive endometrium (n = 12), myometrium (n = 3), and cervix (n = 4). (C) The closest hsa-mir-548 family members to hsa-miR-548ba according to sequence similarity. (D) Hsa-mir-548 family members which share a seed sequence with hsa-miR-548ba. The sequence length in nucleotides is noted after the slash. (E) miRNAs from the hsa-miR-548 family expressed in the myometrium. (F) miRNAs of the hsa-miR-548 family expressed in cumulus and mural granulosa cells. The alignment of hsa-miR-548 family sequences co-expressed with hsa-miR-548ba in the analysed tissues: (G) cumulus granulosa cells; (H) mural granulosa cells; (I) myometrium; (J) The expression of hsa-mir-548 family members in the cell-depleted follicular fluid of the ovarian follicle; (K) the alignment of extracellular miRNAs observed in follicular fluid. Expression levels are displayed as a mean of counts per million (CPM) ± SEM). *p < 0.05, Student’s t-test