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Fig. 2 | BMC Genomic Data

Fig. 2

From: The transcription factor reservoir and chromatin landscape in activated plasmacytoid dendritic cells

Fig. 2

RNA-Seq reveals significant TF expression changes after pDC activation. A Pearson correlation plot for samples used in RNA-Seq. pDCs (CD3CD19CD11c+CD11blowB220+SiglecH+CD317+) were sorted from BM-derived Flt3-L cultures of C57BL/6 N mice and cells were left either naïve or stimulated with CpG for 2 h, 6 h or 12 h. B Volcano plots showing global expression of genes in sorted pDCs at steady state and after 2 h, 6 h, and 12 h of CpG stimulation. TF genes with a |FC| > 2 and a p-value of < 0.05 corrected for the false discovery rate (FDR) were considered significantly differentially expressed and are marked in colour (red and blue). C Bar chart depicting number of DETFs that are up or down-regulated between the respective conditions (|FC| > 2, p < 0.05). D, E Venn diagrams displaying significantly up and down-regulated TF genes (p ≤ 0.05, |FC| ≥ 2) between stimulated pDCs vs naïve pDCs (D) and 12 h pDCs stimulated vs naïve, 2 h and 6 h stimulated pDCs (E). F Heatmap showing normalized expression values (cpm, count per million) of differentially expressed TF genes from (B) in pDCs at steady state and after 2 h, 6 h, and 12 h of CpG stimulation. Hierarchical clustering on rows with average linkage and the One minus Pearson correlation metric was performed

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