Table 2 Primers for full-length cDNA cloning and qRT-PCR
From: Cloning and expression analysis of GATA1 gene in Carassius auratus red var
Primer name | Sequence (5′ → 3′) | Application |
|---|---|---|
GATA1-F1 | GCTCCACAAAAGAAAGTCAT | partial sequence obtaining |
GATA1-R1 | ACGAGGGTGTGTAGAGAAGT | Â |
GATA1-F2 | CCTCAATCACCCACTATGCC | ORF qualifying |
GATA1-R2 | GTGGATTGAGATTCCGACAT | Â |
GATA1-R-out | GCTCTGGCATAGTGGGTGATTGAGGTTA | 5′-Race PCR amplification |
GATA1-R-in | ATAATCGAAACACATCACTCACCCCA | Â |
GATA1-F-out | GGCGTCTACAGCCACTCATCCCATTCAC | 3′-Race PCR amplification |
GATA1-F-in | GGATGCTTTAGTCACACGATGTCGGAAT | Â |
GATA1-qF | CCTTCCCTCCCTTATCCAG | qRT-PCR amplification |
GATA1-qR | GGTAGTGTCCCGTTCCATC | Â |