Fig. 6
From: miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4
Si-ACSL4 decreased lipid droplet accumulation. a Relative mRNA expression levels of ACSL4 in cells transfected with si1-ACSL4, si2-ACSL4, and si3-ACSL4 fragments. b Western blots showing proteins from cells transfected with si3-ACSL4 fragments (GAPDH as the internal reference). c ACSL4 protein expression in cells transfected with si3-ACSL4 fragments (GAPDH as the internal reference). d Oil Red O staining of terminally differentiated adipocytes. e TG levels in the si3-ACSL4 and si3-nc groups. Results are presented as means ± SE; n = 3; Labels (a, b, c) indicate significantly different values (P < 0.05). Labels (A vs. B) indicate extremely significantly different values (P < 0.01)