TY - JOUR AU - Ghosal, Sharmistha AU - Quilloy, Fergie Ann AU - Casal, Carlos AU - Septiningsih, Endang M. AU - Mendioro, Merlyn S. AU - Dixit, Shalabh PY - 2020 DA - 2020/01/17 TI - Trait-based mapping to identify the genetic factors underlying anaerobic germination of rice: Phenotyping, GXE, and QTL mapping JO - BMC Genetics SP - 6 VL - 21 IS - 1 AB - Anaerobic germination is one of the most important traits for rice under direct-seeded conditions. The trait reduces risk of crop failure due to waterlogged conditions after seeding and allows water to be used as a means of weed control. The identification of QTLs and causal genes for anaerobic germination will facilitate breeding for improved direct-seeded rice varieties. In this study, we explored a BC1F2:3 population developed from a cross between BJ1, an indica landrace, and NSIC Rc222, a high-yielding recurrent parent. The population was phenotyped under different screening methods (anaerobic screenhouse, anaerobic tray, and aerobic screenhouse) to establish the relationship among the methods and to identify the most suitable screening method, followed by bulk segregant analysis (BSA) to identify large-effect QTLs. SN - 1471-2156 UR - https://doi.org/10.1186/s12863-020-0808-y DO - 10.1186/s12863-020-0808-y ID - Ghosal2020 ER -