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Fig. 4 | BMC Genomic Data

Fig. 4

From: A simple method to estimate the in-house limit of detection for genetic mutations with low allele frequencies in whole-exome sequencing analysis by next-generation sequencing

Fig. 4

Limit of detection (LOD) of allele frequency in WES analysis. The %RSD values of AFs calculated from independent quadruplicate experiments were plotted against the mean values of AFs (mean of WES-AFs) obtained from their quadruplicate replicates. The trend in the consistency for %RSD vs. mean values of WES-AFs is represented by the 7 point-moving average curve on the graph. The derivation of LOD30%RSD is illustrated by dotted lines (see arrow). All analyses were performed using the following sequencing data sizes: 5 (a), 15 (b), 30 (c), and 40 (d) Gbp

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BMC Genomic Data

ISSN: 2730-6844

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