Table 2 List of selected primer pairs targeting putative B. aethiopum microsatellite loci and assessment of their polymorphism detection ability

MBo01

[AGG]₇

CCTATCCTTCCATCCCGATCG

90

complex, polymorphic

TTGCCGTGAATCAGCCTCAA

MBo02

[ATC]₇

GGGAGAACAAGGATAACAGCAG

115

single locus, monomorphic

TCCATTTCATCACTAGCTCGGT

MBo03

[AGG]₇

CTCCGAGCCCTAGCAACTTT

131

single locus, monomorphic

TCTGGATGACGAAACCTTCACA

MBo04

[ACC]₇

GATGTGGCCGCTCTGATCTC

192

single locus, monomorphic

ACATGCTGGCAAGGTATTCT

MBo05

[AAG]₇

GTCCTAGCACGCTGGCATTA

202

single locus, monomorphic

TGGGTTGCCAATGAACCCTT

MBo06

[ATC]₇

TGGCCATTCAACTGCTTCAC

202

single locus, monomorphic

GAATCTAGCACCAGCAAACCC

MBo07

[AAG]₇

GGCACTGGAGTCCACATCAA

239

single locus, monomorphic

TCCTTCTGTACTGGCATCTCT

MBo08

[AGG]₈

TGATTGTTTCCTCTTCCCTCCT

90

single locus, monomorphic

TTAATGAGCCGAAGAGGAGCC

MBo09

[AGG]₈

TCCCTCACTCCCATCCTCTC

163

single locus, monomorphic

ACTCCACTCCTTCCCTCATACA

MBo10

[AAC]₈

GTTAAAGACGCAGGGCTGGA

166

single locus, monomorphic

CCCACTTAGTGAGATAAGACTTGA

MBo11

[ATC]₈

GCATCACATGGTTTCAGGCT

219

single locus, monomorphic

GCTCAACCATCGGCAGTGTA

MBo12

[ATC]₉

GGAGGAAAGGTTGCCCTAGAA

102

single locus, monomorphic

TCTCAACCTGATGTCATTGCA

MBo13

[AAG]₉

CAGGTTGCATCGGCCCATT

103

complex, polymorphic

GGAGCCTAATGCACCCAGAG

MBo14

[AAC]₉

ATGGCCGATCCCACTTAGTG

117

single locus, monomorphic

GAGAGAACGGCAATAATTTATGCA

MBo15

[AAG]₁₀

GCTGAAGAGGATGAAGAAGAAGC

92

complex, monomorphic

TCATCATCTCCCTCTCCTTCT

MBo16

[AGG]₁₀

CAGCACTGGCCTCACAGC

118

single locus, monomorphic

CCGTCGATCAGTTGTTGGAGA

MBo17

[ATC]₁₀

ACACAATGACCTTTCGCTGA

124

single locus, monomorphic

CCAAACAGGACCTTATGCCA

MBo18

[AAG]₁₀

ACATCCTCTCCTTCATCTCCTT

187

complex, polymorphic

GTTCCTACAATGCTTGGCGC

MBo19

[AAG]₁₀

TGCTATCACCCAATATCTAGGCT

202

single locus, monomorphic

ACAGTCAACAACTACCATACTGC

MBo20

[AAG]₁₀

TGTGGTTAAAGCAATGGAAGCA

229

single locus, monomorphic

GCCGAACTCCTACTCTCATACG

MBo21

[AAG]₁₁

ACAACAGAAGATCAGTATACGTTCT

171

single locus, monomorphic

TTGAGGAATCATGCTTGTCAGT

MBo22

[AAG]₁₄

AGAAGAATTCGGTTAGGTCACAA

108

single locus, monomorphic

AGATAACATGGGTAAGAATTGCCT

MBo23

[AAT]₅

TGAGTTCTTGTCTTGTCTTCGT

100

single locus, monomorphic

GGTTTGGGACACCCTTCAGG

MBo24

[AAT]₉

AAAGTCATGTCTGGGTGATGAA

90

single locus, monomorphic

ATGATGAGCACAGCTACAACTCT

MBo25

[AAT]₆

TCTTCAGGTGACAAGCAACA

96

single locus, monomorphic

CCTGGGCATGGAGATAGCAT

MBo26

[AAT]₇

CCATAGGCCAGCCCACTATA

134

single locus, monomorphic

ACCCTTTCTTCTTCCTCATTTGT

MBo27

[AAT]₇

TCTCTATTGCTTGGTGATCCC

103

single locus, monomorphic

TCCAACAAGGGATGGTTATCATG

MBo28

[AAT]₈

GCCTTGAGAGTGGAAGAGGC

205

single locus, monomorphic

TCTCTTCTTTGCGCCCTCAT

MBo29

[AAT]₁₆

AGACATGTAGAGGTGGGACT

211

single locus, monomorphic

TCTGTATGAGAGACGTGTTACAGT

MBo30

[AAT]₈

TGACCATAACAAGCTACCAGGT

146

single locus, monomorphic

GGTGGAAGCTATTGATATTGCATGT

MBo31

[AAT]₁₀

TGACAATGATGCATGCGATAACA

187

single locus, monomorphic

GCATCACCCATGTCCTTTAGC

MBo32

[AAT]₁₀

TCCGAGGGCAGTATTTGTCG

117

single locus, monomorphic

CACTATTTCGGAAACCTAAGCCC

MBo33

[AAT]₁₇

GCACACTTTGTATCCGACGC

147

single locus, monomorphic

CAGGGATAGTAACCGTCAGGG

MBo34*

[AG]₂₈

GTGGCACCTCTGCGGTTT

192

single locus, polymorphic

CGAGATGGAAGCACCTGGAG

MBo35*

[AG]₂₄

AGCATGCTTTCTGCTTCATGTG

137

single locus, polymorphic

CCTTTCCCTGACTGCATTGC

MBo36

[AG]₂₃

TCGGAAGTCGAATGTGGCAG

180

no amplification

TCGGAAGAGTGGTCAATCATGG

MBo37

[AG]₂₃

GCTCTACTCCCAGAGACGGA

142

complex, polymorphic

AACAGTCGACGGAATGCTCA

MBo38*

[AG]₂₀

AGTCCTCACTGCTGGTGGTA

130

single locus, polymorphic

TCCTTGAATAGTCCATCTTGCA

MBo39

[AG]₁₉

AACGCAGGTTAAGAGGCTCC

168

complex, monomorphic

CCTCCTGGTGCAACCCTTAC

MBo40

[AG]₁₉

TGTGGAGTGTGAGTCGATGG

193

complex, polymorphic

GGCTGCATAATCTCATCACGC

MBo41*

[AG]₁₈

TTCTCCACCAGCCTCACAAC

184

single locus, polymorphic

ATACGGCCCATCAACCCTTC

MBo42

[AG]₁₈

CCTGGTGGTACATGTGGTCA

136

complex, polymorphic

TGTGGCACATTCATTTCTGAAGG

MBo43

[AG]₁₈

AGTTTGTTCTGTGTGTTGTCAC

137

no amplification

GCACACATCTTGCTTTGAAGAC

MBo44

[AG]₁₇

AACACACTTTAAATCGACTTCTTCA

193

complex, polymorphic

CACGGCTGCCATGTGAGG

MBo45

[AG]₁₇

TAGATCGGAAGTCAGGCCC

193

no amplification

AGAGAAGTGGGAGGAGAGGTC

MBo46

[AG]₁₇

GCCGATATTAGCTTCTTCTTGGC

154

single locus, monomorphic

GCCTTGTTGATCCCGTTTCAC

MBo47

[AG]₁₆

GGCACCTGACGCCTCTTT

188

single locus, monomorphic

TCACTTCGACTCAATTGTATCCAT

MBo48

[AG]₁₆

AGGACAAAGAGATGAGAAGCCT

92

complex, polymorphic

ACCAATTCCCAGTTAGTTGACCA

MBo49*

[AG]₁₆

CATCACCCATTCTCTCTGCCT

141

single locus, polymorphic

GAGAAACCATCCGCACCTCA

MBo50*

[AG]₁₅

AGAAGTCATCTTGAGGGCCC

150

single locus, polymorphic

TTGCTAGAATGATACACAAATTGCT

MBo51*

[AG]₁₅

TGTGCTATTTGTTGGGAATGCA

191

single locus, polymorphic

GCAAGCTCATGTTCTAGTTTCAAGT

MBo52*

[AG]₁₅

ACACATCCTACATGAATAGACCTCC

122

single locus, polymorphic

TCTTGTCATAGCCTAGATTCCCT

MBo53

[AG]₁₅

AGGTTTAAGGGTTTGGGTTAGGG

131

single locus, monomorphic

GGTGGAGTAAGTTTGAGGGTCA

MBo54*

[AG]₁₁NNN[AG]₁₅

CATATGCTGATACAAGAGAGAGGG

124

single locus, polymorphic

ACCTTATAAGCAGGATCCAGACA

MBo55

[AG]₁₅

TGGAATCAACCTTGGGTCTACA

198

complex, polymorphic

TCGTCGGTCTTCTAGCCACT

MBo56*

[AG]₁₅

ACCAAGATCAAGCACGAGGA

103

single locus, polymorphic

AGGATCACCCTTTCTTTCTTTCT

MBo57*

[AG]₁₅

GGGTTCAATCCTGATGAGAGCA

136

single locus, polymorphic

ACCGTTCGATCAACCATGGT