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Table 1 Rearing efficiency and female lethality of L. cuprina TSS

From: Building a transgenic sexing strain for genetic control of the Australian sheep blow fly Lucilia cuprina using two lethal effectors

TSS Condition Teta #Pupae #Male #Female #Adult AERb %Male
FL3#2c Heterozygous 2098 644 735 1379 65.7 46.7%
+ 2468 1068 1048 2116 85.1 50.5%
Homozygous 2387 1029 1 1030 43.1 99.9%
+ 1647 672 701 1373 83.3 49.0%
FL3#2d Homozygous 1736 881 2 883 50.9 99.8%
+ 1562 629 685 1314 84.1 47.9%
DH6d (FL3#2; EF1#12) Heterozygous 1717 1383 0 1383 80.5 100%
Homozygous 427 270 0 270 63.2 100%
++/− 1421 885 0 885 62.3 100%
+ 1611 719 669 1388 86.2 51.8%
DH1e (DR2#6; EF1#12) Heterozygous 1091 916 0 916 84 100%
Homozygous +/− 1054 958 0 958 90 100%
+ 2719 1284 1234 2518 92.6 51.0%
DH2e (DR2#6; EF3E) Heterozygous 1252 1099 0 1099 87.8 100%
Homozygous +/− 694 594 0 594 85.6 100%
+ 1724 784 738 1522 88.3 51.5%
DH3f (DR3#2; EF3E) Heterozygous 1082 771 0 771 71.3 100%
Homozygous +/− 1158 981 0 981 84.7 100%
+ 1949 838 810 1648 84.6 50.8%
DH4f (DR3#4; EF1#12) Heterozygous 2398 1690 30 1720 71.7 98.3%
Homozygous +/− 953 801 0 801 84.1 100%
+ 2284 1039 1008 2047 89.6 50.8%
DH5f (DR5#4; EF1#12) Heterozygous 1271 1132 0 1132 89.1 100%
Homozygous 660 561 0 561 85 100%
+ 2162 981 881 1862 86.1 52.7%
  1. a “-” stands for no tetracycline in the diet, and “+” stands for plus tetracycline in the diet, “+/−” indicates parents fed a low dose of tetracycline (1 or 3 μg/mL for the first 2 days), “++/−” indicates a high dose of tetracycline (100 μg/mL) was supplied to the parental adults for the first 8 days but not their progeny that were counted
  2. bAER stands for adult emergence ratio
  3. cData from [20]. Eggs were collected up to two times from 10 to 20 pairs of adults
  4. dData from this study, three replicates of 8-pairs per cage
  5. eData from [12]. For DR2 the bottleneck (bnk) cellularization gene promoter from L. sericata was used to drive expression of tTA. EF3 contains the wild type version of Lshid whereas EF1 has a phosphomutated version called LshidAla2
  6. fData from [31]. The spitting image (spt) gene promoter from L. sericata and the actin5C gene promoter from L. cuprina was used to drive expression of tTA for DR3 and DR5, respectively