|
TSS
|
Condition
|
Teta
|
#Pupae
|
#Male
|
#Female
|
#Adult
|
AERb
|
%Male
|
|---|
|
FL3#2c
|
Heterozygous
|
–
|
2098
|
644
|
735
|
1379
|
65.7
|
46.7%
|
|
+
|
2468
|
1068
|
1048
|
2116
|
85.1
|
50.5%
|
|
Homozygous
|
–
|
2387
|
1029
|
1
|
1030
|
43.1
|
99.9%
|
|
+
|
1647
|
672
|
701
|
1373
|
83.3
|
49.0%
|
|
FL3#2d
|
Homozygous
|
–
|
1736
|
881
|
2
|
883
|
50.9
|
99.8%
|
|
+
|
1562
|
629
|
685
|
1314
|
84.1
|
47.9%
|
|
DH6d (FL3#2; EF1#12)
|
Heterozygous
|
–
|
1717
|
1383
|
0
|
1383
|
80.5
|
100%
|
|
Homozygous
|
–
|
427
|
270
|
0
|
270
|
63.2
|
100%
|
|
++/−
|
1421
|
885
|
0
|
885
|
62.3
|
100%
|
|
+
|
1611
|
719
|
669
|
1388
|
86.2
|
51.8%
|
|
DH1e (DR2#6; EF1#12)
|
Heterozygous
|
–
|
1091
|
916
|
0
|
916
|
84
|
100%
|
|
Homozygous
|
+/−
|
1054
|
958
|
0
|
958
|
90
|
100%
|
|
+
|
2719
|
1284
|
1234
|
2518
|
92.6
|
51.0%
|
|
DH2e (DR2#6; EF3E)
|
Heterozygous
|
–
|
1252
|
1099
|
0
|
1099
|
87.8
|
100%
|
|
Homozygous
|
+/−
|
694
|
594
|
0
|
594
|
85.6
|
100%
|
|
+
|
1724
|
784
|
738
|
1522
|
88.3
|
51.5%
|
|
DH3f (DR3#2; EF3E)
|
Heterozygous
|
–
|
1082
|
771
|
0
|
771
|
71.3
|
100%
|
|
Homozygous
|
+/−
|
1158
|
981
|
0
|
981
|
84.7
|
100%
|
|
+
|
1949
|
838
|
810
|
1648
|
84.6
|
50.8%
|
|
DH4f (DR3#4; EF1#12)
|
Heterozygous
|
–
|
2398
|
1690
|
30
|
1720
|
71.7
|
98.3%
|
|
Homozygous
|
+/−
|
953
|
801
|
0
|
801
|
84.1
|
100%
|
|
+
|
2284
|
1039
|
1008
|
2047
|
89.6
|
50.8%
|
|
DH5f (DR5#4; EF1#12)
|
Heterozygous
|
–
|
1271
|
1132
|
0
|
1132
|
89.1
|
100%
|
|
Homozygous
|
–
|
660
|
561
|
0
|
561
|
85
|
100%
|
|
+
|
2162
|
981
|
881
|
1862
|
86.1
|
52.7%
|
- a “-” stands for no tetracycline in the diet, and “+” stands for plus tetracycline in the diet, “+/−” indicates parents fed a low dose of tetracycline (1 or 3 μg/mL for the first 2 days), “++/−” indicates a high dose of tetracycline (100 μg/mL) was supplied to the parental adults for the first 8 days but not their progeny that were counted
- bAER stands for adult emergence ratio
- cData from [20]. Eggs were collected up to two times from 10 to 20 pairs of adults
- dData from this study, three replicates of 8-pairs per cage
- eData from [12]. For DR2 the bottleneck (bnk) cellularization gene promoter from L. sericata was used to drive expression of tTA. EF3 contains the wild type version of Lshid whereas EF1 has a phosphomutated version called LshidAla2
- fData from [31]. The spitting image (spt) gene promoter from L. sericata and the actin5C gene promoter from L. cuprina was used to drive expression of tTA for DR3 and DR5, respectively
