Hosta | Vector | G0 eggs injected | G0s matedb | No. G0 lines | No. fertile G0 lines | No. G1 transformant lines | Transformation frequencyc |
---|
Dm | pKhop [Dmwhite+] | 436 | 131 | 98 | 79 | 2 | 0.025 |
Dm | phop [PUb-DsRed.T3] | 1056 | 203 | 170 | 129 | 2 | 0.016 |
As | phop [PUb-DsRed.T3] | 1453 | 389 | 103 | 94 | 9 | 0.096 |
- aDm: D. melanogaster w[m] strain; As: A. suspensa wild type strain
- btotal number of G0 adults mated based on 70, 140, and 80 single G0 adult matings (to 3 opposite sex adults) for D. melanogaster pKhop[Dmwhite+] and phop[PUb-DsRed.T3] transformations, and the A. suspensa phop[PUb-DsRed.T3] transformation, respectively; additional G0s were mated in groups of 2–3 same sex G0 adults to opposite sex adults
- ctransformation frequency calculated as number of transformant lines per number of fertile lines