The hAT-family transposable element, hopper, from Bactrocera dorsalis is a functional vector for insect germline transformation

Table 1 hopper^Bd-we element transformation experiments in D. melanogaster and A. suspensa

Host^a	Vector	G₀ eggs injected	G₀s mated^b	No. G₀ lines	No. fertile G₀ lines	No. G₁ transformant lines	Transformation frequency^c
Dm	pKhop [Dmwhite+]	436	131	98	79	2	0.025
Dm	phop [PUb-DsRed.T3]	1056	203	170	129	2	0.016
As	phop [PUb-DsRed.T3]	1453	389	103	94	9	0.096

^aDm: D. melanogaster w[m] strain; As: A. suspensa wild type strain
^btotal number of G₀ adults mated based on 70, 140, and 80 single G₀ adult matings (to 3 opposite sex adults) for D. melanogaster pKhop[Dmwhite⁺] and phop[PUb-DsRed.T3] transformations, and the A. suspensa phop[PUb-DsRed.T3] transformation, respectively; additional G₀s were mated in groups of 2–3 same sex G₀ adults to opposite sex adults
^ctransformation frequency calculated as number of transformant lines per number of fertile lines

ISSN: 2730-6844