Hosta
|
Vector
|
G0 eggs injected
|
G0s matedb
|
No. G0 lines
|
No. fertile G0 lines
|
No. G1
transformant lines
|
Transformation
frequencyc
|
---|
Dm
|
pKhop [Dmwhite+]
|
436
|
131
|
98
|
79
|
2
|
0.025
|
Dm
|
phop [PUb-DsRed.T3]
|
1056
|
203
|
170
|
129
|
2
|
0.016
|
As
|
phop [PUb-DsRed.T3]
|
1453
|
389
|
103
|
94
|
9
|
0.096
|
- aDm: D. melanogaster w[m] strain; As: A. suspensa wild type strain
- btotal number of G0 adults mated based on 70, 140, and 80 single G0 adult matings (to 3 opposite sex adults) for D. melanogaster pKhop[Dmwhite+] and phop[PUb-DsRed.T3] transformations, and the A. suspensa phop[PUb-DsRed.T3] transformation, respectively; additional G0s were mated in groups of 2–3 same sex G0 adults to opposite sex adults
- ctransformation frequency calculated as number of transformant lines per number of fertile lines