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Table 1 hopperBd-we element transformation experiments in D. melanogaster and A. suspensa

From: The hAT-family transposable element, hopper, from Bactrocera dorsalis is a functional vector for insect germline transformation

Hosta

Vector

G0 eggs injected

G0s matedb

No. G0 lines

No. fertile G0 lines

No. G1

transformant lines

Transformation

frequencyc

Dm

pKhop [Dmwhite+]

436

131

98

79

2

0.025

Dm

phop [PUb-DsRed.T3]

1056

203

170

129

2

0.016

As

phop [PUb-DsRed.T3]

1453

389

103

94

9

0.096

  1. aDm: D. melanogaster w[m] strain; As: A. suspensa wild type strain
  2. btotal number of G0 adults mated based on 70, 140, and 80 single G0 adult matings (to 3 opposite sex adults) for D. melanogaster pKhop[Dmwhite+] and phop[PUb-DsRed.T3] transformations, and the A. suspensa phop[PUb-DsRed.T3] transformation, respectively; additional G0s were mated in groups of 2–3 same sex G0 adults to opposite sex adults
  3. ctransformation frequency calculated as number of transformant lines per number of fertile lines