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Fig. 3 | BMC Genetics

Fig. 3

From: Targeting the autosomal Ceratitis capitata transformer gene using Cas9 or dCas9 to masculinize XX individuals without inducing mutations

Fig. 3

RT-PCR analysis of reverted XX males. (A) The six XX males from injection set n. 2 (Table 1) showed only male-specific Cctra transcripts (500 bp) and shorter less abundant splicing variants. No traces of female-specific transcripts were visible in the gel electrophoresis (160 bp) (B) RT-PCR with MoY-specific primers indirectly confirmed the absence of the Y chromosome in these six fully reverted XX males and its presence in XY flies (positive control)

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BMC Genomic Data

ISSN: 2730-6844

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