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Fig. 4 | BMC Genetics

Fig. 4

From: Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii

Fig. 4

D. suzukii nanos and the use of its promoter/enhancer for directed expression. a Schematic representation of the D. suzukii gene nanos. b Whole mount in situ hybridisation to detect nanos expression in wildtype D. suzukii ovaries. c piggyBac-based transgenic construct HMMA223 to generate φC31 integrase RMCE self-docking lines. RMCE in a self-docking line, which provides both the recombination target sequences attP as well as the φC31 integrase driven by the nanos promoter/enhancer providing maternal expression, will result in marker exchange as well as cargo gene (GOI) integration and removal of the integrase source. d Whole mount in situ hybridisation to detect φC31 integrase expression in transgenic D. suzukii ovaries carrying construct HMMA223. Expression of nanos or φC31 integrase, respectively, is detected in the nurse cells of the ovaries

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