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Fig. 2 | BMC Genetics

Fig. 2

From: Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii

Fig. 2

Spermatogenesis-specific driver for binary tet-off expression system. a piggyBac-based transgenic construct HMMA389 to generate a testes-specific driver line carrying the β2t promoter [52] fused to tTA. b-d Whole mount in situ hybridisation to detect gene expression in D. suzukii male reproductive organs. b Testes-specific tTA expression driven by the Ds_β2t promoter in line 389_F25M1. c Ds_β2t expression in wildtype testes detected by an antisense probe. d Negative control using a Ds_β2t sense probe on wildtype testes

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