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Fig. 4 | BMC Genetics

Fig. 4

From: Identification and characterization of four Drosophila suzukii cellularization genes and their promoters

Fig. 4

Functional characterization of Drosophila suzukii cellularization gene promoters. (a) Schematic map of the 5′ flanking sequences from four cellularization genes (Dssry-α, Dsnullo, Dsslam and Dsbnk). The positions of TAGteam motifs, conserved motifs (CM), TATA box and 5′ UTR were indicated. (b) piggyBac test vectors V205, V206, V207 and V208, in which the Dssry-α, Dsnullo, Dsslam and Dsbnk promoters, are used to drive the expression of DsRed with a nuclear localization signal (NLS), respectively. All test vectors contain an AmCyan marker gene regulated by the constitutive D. melanogaster polyubiquitin (DmPUb) promoter and an attP recombination site. (c) Drosophila S2 cells were transfected with piggyBac vectors and images taken under epifluorescent light conditions using the FITC filter for AmCyan or CY5 filter for DsRed. (d) The number of cells that show red and blue fluorescence was counted using Image J, and the ratio of red to blue cells was calculated. Each bar presents the mean ± SE of n = 3 experiments. The bars with different letters are significantly different at P < 0.05 (one-way ANOVA, Duncan’s multiple range test)

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