Fig. 5From: Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoniGenetic crosses performed to determine ratio of progeny carrying shits1 mutant allele(s). Crosses were carried out over three generations (G1-G3 matings) and the G2-G4 adult progeny were genotyped to determine if they carried any shits1 mutant allele(s). The observed ratio of progeny from each mating was compared to the expected ratioBack to article page