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Fig. 2 | BMC Genetics

Fig. 2

From: Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoni

Fig. 2

CRISPR guide sequence used to introduce the shits1 point mutation into B. tryoni. The single base change that is being induced in the shits1 locus is a ‘G’ to ‘A’, resulting in change of the amino acid residue glycine (G, highlighted by the red box) to aspartic acid and removing the BaeGI restriction site (GKGCM^C). The red arrow indicates the PAM cut site

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BMC Genomic Data

ISSN: 2730-6844

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