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Fig. 5 | BMC Genetics

Fig. 5

From: Functional identification of PsMYB57 involved in anthocyanin regulation of tree peony

Fig. 5

Transcript levels of structural anthocyanin genes and endogenous bHLH genes in wild-type and transgenic tobacco. a to f are transcription levels of NtCHS, NtF3’H, NtDFR and NtANS, NtAn1a and NtAn1b in leaves, sepals and pericarps of wild-type and transgenic tobacco lines. Transcription level of NtAn1a was not detectable in wild-type lines, and transgenic line 3 used as control in (e). CK was wild-type tobacco lines, Line 1 to Line 3 were three PsMYB57 transgenic tobacco lines. Three T1 generation lines from each transgenic tobacco were collected as samples. TubulinA1 was used as an internal control to normalize the relative expression levels of the analyzed genes. qRT-PCR analysis was performed with three biological and technical replicates per experiment. An asterisk indicates statistical significance (P < 0.05)

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