Fig. 1From: Functional analysis of bovine interleukin-10 receptor alpha in response to Mycobacterium avium subsp. paratuberculosis lysate using CRISPR/Cas9a Gel image of Genomic Cleavage Detection Assay using Bovine MAC-T transfected cells. Detection of two bands (upper parental 645 bp band and lower 493 bp cleavage band - shown with arrows) in transfected cells (T) as opposed to one parental band in control non-transfected cells (C) indicates gene editing in IL10RA by specific sgRNAs. b Aligned sequences of exon 3 of IL-10RA gene of knockout (KO) MAC-T cells with reference sequence showing 13 bp deletion using CLC Genomics WorkbenchBack to article page