Table 2 The expression stability of candidate reference genes in tissues and cultured cells of different ploidy fish assessed by the Bestkeeper software
Gene | Tissues | Cultured cells | ||||
|---|---|---|---|---|---|---|
SD | CV | Rank | SD | CV | Rank | |
RPS5 | 1.06 | 6.24 | 2 | 0.38 | 2.19 | 2 |
RPS18 | 0.18 | 4.75 | 1 | 0.4 | 2.45 | 3 |
RPL7 | 1.15 | 6.58 | 3 | 0.44 | 2.73 | 4 |
RPLP2 | 1.3 | 5.7 | 6 | 0.78 | 3.5 | 7 |
RPL13α | 1.23 | 6.61 | 4 | 0.57 | 3.15 | 6 |
EF1-α | 1.3 | 7.85 | 5 | 0.3 | 1.9 | 1 |
DDX5 | 1.4 | 7.04 | 7 | 0.56 | 2.79 | 5 |
β-actin | 1.54 | 9.24 | 8 | 1.46 | 9.58 | 9 |
β-tubulin | 1.76 | 8.11 | 9 | 1.54 | 7.54 | 10 |
hprt1 | 1.87 | 8.22 | 10 | 0.8 | 3.77 | 8 |
B2M | 4.83 | 20.71 | 12 | 3.49 | 16.19 | 12 |
GAPDH | 3.91 | 18.34 | 11 | 2.05 | 6.95 | 11 |