Fig. 4

Maximum likelihood phylogenetic tree for the amino acid sequences of MHC-DOB exon 2. This tree contains translated sequences for white-tailed deer (Odvi, Odocoileus virginianus; MK952691- MK952701), cow (Bola, Bos taurus; 282493; [112]), sheep (Ovar, Ovis aries; Z49879.1; [107]), and red deer (Ceel, Cervus elaphus; CM008014.1; [7]). It was rooted with the cow outgroup. Node labels are standard bootstrap support (%). Arrows indicate the presence of near-zero internal branch lengths (< 0.011), which should be interpreted with caution. Heatmap colors indicate all white-tailed deer MHC-DOB alleles and further correspond to Odvi-DOB allele frequencies found in our population, where red is the most common translated MHC-DOB allele for MHC-DOB exon 2 in white-tailed deer, though both are quite rare