Table 2 Primer sequences of the target fragments
From: Effects of HTR1B 3′ region polymorphisms and functional regions on gene expression regulation
Primer | Length | Sequences |
|---|---|---|
Common end (+ 1754)-F | 5′ CCGCTCGAGAAACTGATACGTTTTAAGTG 3’ | |
D0 (+ 1754 ~ + 3285)-R | 1532 bp | 5′ GCTCTAGAGCGTTTCCTGATTGTTAGTAAGT 3’ |
D1 (+ 1754 ~ + 3143)-R | 1390 bp | 5′ GCTCTAGAGCTTCTCCTGCCCAAACTTC 3’ |
D2 (+ 1754 ~ + 3008)-R | 1255 bp | 5′ GCTCTAGAGCTATGTTAGCACACAAGGAATC 3’ |
D3 (+ 1754 ~ + 2887)-R | 1134 bp | 5′ GCTCTAGAGCCCATTCCTCAATTGTGTAAG 3’ |
D4 (+ 1754 ~ + 2769)-R | 1016 bp | 5′ GCTCTAGAGCTCGGTTTTACCAATTGCAT 3’ |
D5 (+ 1754 ~ + 2440)-R | 687 bp | 5′ GCTCTAGAGCTCTCAGCATCAGAATTTTG 3’ |
D6 (+ 1754 ~ + 2311)-R | 558 bp | 5′ GCTCTAGAGCAATGATGCCAAAGTAACTGTT 3’ |
D7 (+ 1754 ~ + 1953)-R | 200 bp | 5′ GCTCTAGAGCTATTCTGGCTTCTCAGGATC 3’ |