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Fig. 4 | BMC Genetics

Fig. 4

From: Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean

Fig. 4

Expression levels of selected defense-responsive genes in transgenic soybean in response to P. sojae inoculation. Total RNA was extracted from fully grown leaves of T3 transgenic plants 0, 1, 2, 4, 8, 12, and 24 h after inoculation with P. sojae race 1 mycelia. Expression level of each gene was quantified relative to that of the internal control (GmACT) using the 2-ΔΔCt method. Data were represented as the mean of three biological replicates, with error bars indicating standard errors. (A) to (H) indicated the expression levels of SA-dependent pathway genes GmPR1, GmPR2, GmPR3, GmPR5, GmPR12, and GmPAL, respectively; (I) and (J) indicate the expression levels of SA-dependent pathway genes GmAOS and GmPPO, respectively; (K) and (L) indicated plant disease resistance genes GmSGT1 and GmRAR1, respectively. WT, wild-type Williams 82; 8096 and 8253, T3 transgenic lines

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