Fig. 1

Schematic representation of the plant expression vector and screening of transgenic soybean. (A) Schematic representation of the recombinant plasmid pCambia3300-CaMV 35S-CaAMP1. RB and LB, the right and left borders of the T-DNA, respectively. CaAMP1 and bar inserts were both driven by the CaMV 35S promoter. Bold arrows indicated the primer binding sites of CaAMP1-F1/R1. The solid bar represented the PCR product using Bar-F/R. (B) LibertyLink strip analysis of the T0 transgenic plants. WT, wild-type Williams 82; 1–13, transgenic soybean lines. (C) PCR analysis of T3 transgenic lines using CaAMP1-F1/R1 and Bar-F/R primers. M, DL2000 marker; Ctl+, positive plasmid; WT, wild-type Williams 82; numbers 1–6, transgenic soybean lines 8096, 8101, 8111, 8130, 8197, and 8253, respectively. (D) Southern blot analysis of the T3 transgenic lines using CaAMP1 as a probe. M, 15-kb DNA marker; Ctl+, positive plasmid; WT, wild-type Williams 82; 1–6, transgenic lines 8096, 8101, 8111, 8130, 8197, and 8253, respectively