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Table 3 Analysis of differences in locomotor activity factored by age and conditions using two-way ANOVA

From: Amyloid-β peptides slightly affect lifespan or antimicrobial peptide gene expression in Drosophila melanogaster

Variant

Source of variation

SS

DF

MS

F

P-value

F-crit

males

 pS8-Aβ42

Condition

4231.89

1

4231.89

0.918

0.344

7.396

Age

3,031,998.79

5

606,399.76

131.572

4.4e-22

3.574

Interaction

25,648.17

5

5129.63

1.113

0.371

3.574

Within (errors)

165,919.81

36

4608.88

   

Total

3,227,798.65

47

    

 Aβ42

Condition

3615.74

1

3615.74

0.623

0.435

7.396

Age

3,237,235.33

5

647,447.07

111.585

7.1e-21

3.574

Interaction

34,015.75

5

6803.15

1.172

0.342

3.574

Within (errors)

208,882.47

36

5802.29

   

Total

3,483,749.29

47

    

 isoD7-Aβ42

Condition

46,787.54

1

46,787.54

4.357

0.044

7.396

Age

4,055,146.09

5

811,029.22

75.526

4.6e-18

3.574

Interaction

17,717.56

5

3543.51

0.33

0.892

3.574

Within (errors)

386,580.72

36

10,738.35

   

Total

4,506,231.91

47

    

females

 pS8-Aβ42

Condition

148.05

1

148.05

0.32

0.575

7.396

Age

357,497.08

5

71,499.42

154.507

2.8e-23

3.574

Interaction

1593.83

5

318.77

0.689

0.635

3.574

Within (errors)

16,659.28

36

462.76

   

Total

375,898.24

47

    

 Aβ42

Condition

3605.33

1

3605.33

6.954

0.012

7.396

Age

383,645

5

76,729

147.986

5.9e-23

3.574

Interaction

3418.1

5

683.62

1.318

0.278

3.574

Within (errors)

18,665.55

36

518.49

   

Total

409,333.98

47

    

 isoD7-Aβ42

Condition

3918.66

1

3918.66

4.696

0.037

7.396

Age

338,379.79

5

67,675.96

81.098

1.4e-18

3.574

Interaction

5280.92

5

1056.18

1.266

0.299

3.574

Within (errors)

30,041.99

36

834.5

   

Total

377,621.36

47

    
  1. SS Sum-of-squares, DF degrees of freedom, MS mean squares. Results of F-tests: F-value (F), P-value, F-critical value (F-crit). Significant estimates in bold