Fig. 5From: Cloning of Hynobius lichenatus (Tohoku hynobiid salamander) p53 and analysis of its expression in response to radiationWestern blot analysis of Hynobius lichenatus proteins in mammalian cells. The expression plasmids (phyβ-actin-HA, phyGAPDH-HA, and phyp53-HA) were transfected into HEK293 cells. Transgene expression was analyzed by western blotting two days after transfection using antibodies against the HA-tag. The original western blot image is shown in Additional file 5ABack to article page