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Fig. 3 | BMC Genetics

Fig. 3

From: Sex determination in the GIFT strain of tilapia is controlled by a locus in linkage group 23

Fig. 3

Amplified PCR products on 1.5% agarose gel using markers from the different regions in the variations of the Amh gene for 50 GIFT and 4 Stirling broodstock. a Deletion in Amh exon VII (AmhΔy [12]). A 439 bp band was evident in all individuals and an extra 206 bp band was present in nearly all males. b Insertion in Amh exon VI (AmhΔy [12]); a 547 bp band was present in nearly all sires and dams showed no band (Except 1D). c Exon 0 deletion (161 bp) in AmhΔy [12]; a 547 bp band was present in all individuals except 22S (purple circle, putative YY) and nearly all males showed a band with 386 bp (had 161 bp deletion). The other exceptions to the consensus patterns are: red circle indicates dam and sire concluded to be wrongly labelled (transposed) individuals, green circle indicates sire concluded to be an XX neo-male, progeny information was available for blue circled sire but the dam information was not available. M - molecular marker (100 bp), number = family number from Supplementary Table S1; D - dam, S – sire, DW - distilled water. Stirling broodstocks were non-informative

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