Table 4 Sequences of primers used in this study
From: Glyoxalase 1 gene improves the antistress capacity and reduces the immune inflammatory response
Primer | Sequence | Usage |
|---|---|---|
G1S | CCACGCCTTACTGAAGCAGGCAAG | Glo1 amplification |
G1A | CCTGAGGTCTAAATCACCTG | Â |
G2S | GCCAGGACTAGCCAAATTC | Glo1 amplification |
G2A | CAGCTGGCACTCACCT | Â |
G3S | TACACACGAATCCTTGGAATG | Glo1 amplification |
G3A | GTGGAGTGGAGTTGCCTCCGCTG | Â |
G4S | CTAGGAAGAGGCTACCTTTGGC | Glo1 amplification |
G4A | CTTGCAGGCAGCATACACATCTG | Â |
G5S | TGCTGTATTGCTCCTGTTAC | Glo1 amplification |
G5A | CTTGCAGGCAGCATACACATCTG | Â |
G6S | GTTTGCTCTCTGTAGGCCACA | Glo1 amplification |
G6A | CACATATCACCAGTTCTCGTTACC | Â |
173S | CATTCCTCCAGAACCCCAGTAGTC | SNP genotyping |
173A | TGTGTGGCACCAAAGCCTCTAGTT | Â |
Glo1-F | AAGACAGCCTGGACCTTCTC | Probe amplification |
Glo1-R | ACGTGGGTCTGAGTTTCCAT | Â |
40S | ACATGGTGTCCATCTGCTCGTC | Glo1 RT-qPCR |
40A | TCGTTACCCTCTCCCACTAGTTTTT | Â |
B1 | AACGGATCCGGTATGTGCAAAGC’ | Beta-actin RT-qPCR |
B2 | GGGTCAGGATACCTCTCTTGCTCTG | Â |