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Fig. 1 | BMC Genetics

Fig. 1

From: Bos taurus–indicus hybridization correlates with intralocus sexual-conflict effects of PRDM9 on male and female fertility in Holstein cattle

Fig. 1

The putative amino-acid variation encoded in exon 10 of PRDM9. Genomic DNA of sires preferentially homozygous for haplotype alleles were used as templates for amplification and for Sanger sequencing using nucleotide primers 1 and 2 in Additional file 1: Table S1. The resulting traces were compared to reference sequences (Bos taurus [GenBank: NP_001306826.2] and two variants of B. indicus based on sequence phasing [GenBank: XP_019820291.1 and ANN45578]) or assembled sequences from NGS data (Holstein, JJ [ENA: LR536713]). The amino-acid alignment was colored using Boxshade. Dashes indicate gaps introduced by the alignment program or stop codons. Identical and similar amino acid residues in at least two of four sequences are indicated by a black and gray background, respectively. White boxes indicate non-conservative amino acid changes between the proteins. Above the alignment, tandem ZnF repeats are labeled following Zhou et al., 2018 [15]. Each repeat consists of 28 residues. Nomenclature of variants follows that of their associated BTA1 telomeric SNP haplotypes (Table 3) and theirs DNA encoding sequences (Additional file 1)

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