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Table 2 Summary of bulked GbS data, GbS read processing and variant detection

From: Rapid identification of inflorescence type markers by genotyping-by-sequencing of diploid and triploid F1 plants of Hydrangea macrophylla

  Diploid pool Mophead pool
Description of pooled plants 99 diploid F1 plants independent of inflorescence type 65 diploid and triploid mophead F1 plants
Total number of high-quality read pairs used for read mapping 92,144,015 58,117,508
Total number of mapped reads 173,568,387
(94.2%)
109,338,937
(94.1%)
Number of contigs with read matches 603,104
(39.7%)
527,059
(34.7%)
Number of reads per scored contig 1 to 765,278 1 to 367,469
Sum of covered nucleotides of the draft genome 248.9 million (15.3%) 196.0 million (12.1%)
Average coverage excluding zero coverage regions (± standard deviation) 98.7 ± 596.76 78.9 ± 388.36
Total number of polymorphic calls with allele frequencies between 18 and 100% 1,603,884 1,808,537
Total number of polymorphic positions with allele frequencies between 25 and 75% indicating heterozygosity 606,317 n.d.