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Table 2 Associations of six SNPs in PRKG1 gene with milk fatty acid traits (LSM ± SE)

From: A post-GWAS confirming effects of PRKG1 gene on milk fatty acids in a Chinese Holstein dairy population

SNP Genotype (No.) C6:0 (%) C8:0 (%) C10:0 (%) C11:0 (%) C12:0 (%) C13:0 (%) C14:0 (%) C14:1 (%) C15:0 (%) C16:0 (%) C16:1 (%) C17:0 (%)
 g.8344262A > T AA(424–459) 0.4901 ± 0.0122 0.9658 ± 0.0107 2.8472 ± 0.0321Aa 0.0577 ± 0.0023 2.9861 ± 0.0418 0.0988 ± 0.0028 10.2891 ± 0.0702 0.6447 ± 0.0180 0.9972 ± 0.0122 34.7950 ± 0.1771 1.3324 ± 0.0248ab 0.5664 ± 0.0031
AT(259–393) 0.4735 ± 0.0125 0.9467 ± 0.0109 2.7685 ± 0.0332Bb 0.0581 ± 0.0025 2.9584 ± 0.0423 0.0991 ± 0.0030 10.1625 ± 0.0716 0.6598 ± 0.0187 0.9947 ± 0.0127 34.7954 ± 0.1822 1.3529 ± 0.0257a 0.5714 ± 0.0032
TT(96–104) 0.4769 ± 0.0174 0.9366 ± 0.0150 2.8580 ± 0.0438ABa 0.0559 ± 0.0035 2.9889 ± 0.0572 0.0965 ± 0.0047 10.3280 ± 0.0951 0.6555 ± 0.0271 0.9880 ± 0.0188 34.9466 ± 0.2566 1.2643 ± 0.0356b 0.5709 ± 0.0047
P 0.2405 0.0177 0.0012** 0.8124 0.6385 0.8615 0.0297 0.6391 0.8733 0.7983 0.0295 0.1829
 g.6904047G > T GG(420–458) 0.4580 ± 0.0133 0.9292 ± 0.0119 2.8354 ± 0.0350 0.0580 ± 0.0026 3.0006 ± 0.0449 0.0988 ± 0.0032 10.2605 ± 0.0777a 0.6579 ± 0.0199 0.9950 ± 0.0133 34.8344 ± 0.1923 1.2995 ± 0.0272a 0.5621 ± 0.0034
GT(179–196) 0.4682 ± 0.0155 0.9361 ± 0.0131 2.8250 ± 0.0394 0.0584 ± 0.0030 2.9910 ± 0.0502 0.0987 ± 0.0039 10.0941 ± 0.0865b 0.6642 ± 0.0229 1.0011 ± 0.0159 34.9128 ± 0.2225 1.3677 ± 0.0312b 0.5678 ± 0.0040
TT(21–24) 0.5161 ± 0.0316 0.8947 ± 0.0258 2.8551 ± 0.0727 0.0538 ± 0.0064 3.0339 ± 0.0974 0.0947 ± 0.0085 10.1180 ± 0.1720ab 0.6469 ± 0.0489 0.9967 ± 0.0346 34.7830 ± 0.4581 1.3050 ± 0.0656ab 0.5592 ± 0.0085
P 0.1453 0.2466 0.8812 0.7751 0.8935 0.8846 0.0377 0.9145 0.9096 0.9003 0.0304 0.2151
 g.6903810G > A AA(17–21) 0.5425 ± 0.0347 1.0524 ± 0.0269A 2.7307 ± 0.0780 0.0547 ± 0.0070 2.8806 ± 0.1040 0.0922 ± 0.0096 10.3798 ± 0.1808 0.6550 ± 0.0523 0.9345 ± 0.0366 34.3377 ± 0.4963 1.2467 ± 0.0677 0.5534 ± 0.0091
AG(179–191) 0.4720 ± 0.0146 0.9352 ± 0.0127B 2.7669 ± 0.0373 0.0580 ± 0.0029 2.9549 ± 0.0484 0.0977 ± 0.0037 10.2389 ± 0.0820 0.6663 ± 0.0225 1.0024 ± 0.0153 34.8727 ± 0.2159 1.3247 ± 0.0301 0.5686 ± 0.0039
GG(681–741) 0.4849 ± 0.0114 0.9418 ± 0.0102B 2.8293 ± 0.0309 0.0592 ± 0.0022 3.0072 ± 0.0393 0.1001 ± 0.0026 10.2294 ± 0.0664 0.6561 ± 0.0169 0.9969 ± 0.0114 34.7215 ± 0.1659 1.3250 ± 0.0233 0.5674 ± 0.0029
P 0.1040 <.0001** 0.0464 0.7411 0.1944 0.6019 0.6868 0.8697 0.1831 0.4741 0.4903 0.2478
 g.6903365C > A AA(93–107) 0.4439 ± 0.0206Aa 0.9343 ± 0.0169A 2.8432 ± 0.0489 0.0586 ± 0.0041 3.0238 ± 0.0635 0.1018 ± 0.0053 10.4947 ± 0.1101 0.6549 ± 0.0305 1.0037 ± 0.0211 34.7232 ± 0.2894 1.2476 ± 0.0410 0.5653 ± 0.0053
CC(361–381) 0.5229 ± 0.0138Bb 0.9924 ± 0.0122B 2.8967 ± 0.0367 0.0592 ± 0.0027 3.0341 ± 0.0471 0.0997 ± 0.0033 10.3851 ± 0.0799 0.6661 ± 0.0206 0.9927 ± 0.0140 34.9631 ± 0.2004 1.3330 ± 0.0283 0.5662 ± 0.0035
CA(49–55) 0.4626 ± 0.0240ABa 0.9379 ± 0.0196A 2.8406 ± 0.0566 0.0576 ± 0.0048 2.9559 ± 0.0745 0.1039 ± 0.0063 10.1970 ± 0.1278 0.6478 ± 0.0365 0.9746 ± 0.0254 34.2494 ± 0.3514 1.2822 ± 0.0488 0.5598 ± 0.0063
P <.0001** <.0001** 0.2980 0.9415 0.5068 0.7647 0.0958 0.8336 0.6052 0.0875 0.0569 0.5867
 g.6902878 T > G GG(189–202) 0.4323 ± 0.0169A 0.9624 ± 0.0144Aa 2.8052 ± 0.0423 0.0559 ± 0.0033 2.9744 ± 0.0550 0.0973 ± 0.0042 10.1691 ± 0.0957a 0.6547 ± 0.0252 0.9966 ± 0.0172 34.7964 ± 0.2433 1.3831 ± 0.0341 0.5667 ± 0.0044
GT(2) 0.7892 ± 0.0983B 1.3588 ± 0.0820B 3.1626 ± 0.2348 0.0560 ± 0.0212 3.0685 ± 0.3132 0.0971 ± 0.0288 11.5294 ± 0.5289b 0.5671 ± 0.1627 0.9937 ± 0.1135 34.7693 ± 1.5091 1.1787 ± 0.2088 0.5358 ± 0.0283
TT(306–334) 0.4942 ± 0.0161C 0.9916 ± 0.0137Ab 2.8670 ± 0.0409 0.0595 ± 0.0032 3.0380 ± 0.0527 0.1015 ± 0.0040 10.2332 ± 0.0918a 0.6368 ± 0.0243 1.0049 ± 0.0166 34.8385 ± 0.2325 1.3627 ± 0.0326 0.5671 ± 0.0042
P <.0001** <.0001** 0.0426 0.4274 0.2875 0.5502 0.0249 0.6194 0.8525 0.9768 0.4788 0.5390
 g.6901713 T > G GG(21–27) 0.5161 ± 0.0328AB 1.0577 ± 0.0246A 2.8817 ± 0.0710AB 0.0539 ± 0.0064 2.8762 ± 0.0928 0.1008 ± 0.0083 10.0821 ± 0.1607ab 0.5555 ± 0.0474 0.9906 ± 0.0333 34.1191 ± 0.4451 1.3422 ± 0.0617 0.5699 ± 0.0083
GT(266–289) 0.4958 ± 0.0132A 0.9797 ± 0.0116B 2.9305 ± 0.0345A 0.0579 ± 0.0026 3.0584 ± 0.0448 0.1000 ± 0.0033 10.3430 ± 0.0756a 0.6518 ± 0.0201 0.9971 ± 0.0137 34.8534 ± 0.1956 1.3099 ± 0.0273 0.5649 ± 0.0035
TT(591–637) 0.4628 ± 0.0116B 0.9230 ± 0.0103C 2.8342 ± 0.0310B 0.0579 ± 0.0023 3.0024 ± 0.0397 0.0982 ± 0.0027 10.1984 ± 0.0673b 0.6604 ± 0.0171 0.9930 ± 0.0115 34.9830 ± 0.1682 1.3285 ± 0.0237 0.5711 ± 0.0030
P 0.0029* <.0001** 0.0004** 0.8083 0.0545 0.8097 0.0181 0.0734 0.9357 0.1082 0.6493 0.1080
SNP Genotype (No.) C17:1 (%) C18:0 (%) C18:1cis-9 (%) C18index (%) C20:0 (%) C14index (%) C16index (%) C17index (%) SFA (%) UFA (%) SFA/UFA (%) Total index (%)
 g.8344262A > T AA(374–460) 0.1906 ± 0.0025a 14.0884 ± 0.0864 18.9343 ± 0.1167a 56.9012 ± 0.2735ab 0.1685 ± 0.0017 6.0733 ± 0.1384 3.6217 ± 0.0636AB 24.8838 ± 0.2156a 68.1683 ± 0.1617 30.2498 ± 0.1462 2.2891 ± 0.0212 27.5332 ± 0.1278A
AT(331–393) 0.1957 ± 0.0026b 14.0342 ± 0.0907 19.2495 ± 0.1212b 57.3243 ± 0.2805a 0.1706 ± 0.0017 6.2370 ± 0.1435 3.6885 ± 0.0659A 24.9935 ± 0.2245a 67.9305 ± 0.1671 30.4415 ± 0.1520 2.2709 ± 0.0221 27.9090 ± 0.1313B
TT(82–104) 0.1889 ± 0.0036ab 14.2026 ± 0.1381 18.7636 ± 0.1828a 56.3603 ± 0.4109b 0.1699 ± 0.0027 6.1182 ± 0.2045 3.4344 ± 0.0913B 24.1599 ± 0.3177b 68.4212 ± 0.2469 29.9512 ± 0.2243 2.3224 ± 0.0329 27.1715 ± 0.1948A
P 0.0218 0.4469 0.0031* 0.0323 0.3960 0.3704 0.0101 0.0154 0.0770 0.0628 0.2492 <.0001**
 g.6904047G > T GG(382–459) 0.1909 ± 0.0027Aa 14.0083 ± 0.0952 19.0595 ± 0.1295 57.3442 ± 0.2994 0.1668 ± 0.0020a 6.2722 ± 0.1517 3.6249 ± 0.0698 24.9868 ± 0.2379A 68.0210 ± 0.1771 30.3843 ± 0.1611 2.2734 ± 0.0232 27.7337 ± 0.1392
GT(173–196) 0.1951 ± 0.0031ABa 14.0877 ± 0.1147 19.0396 ± 0.1519 56.9780 ± 0.3490 0.1718 ± 0.0023b 6.3520 ± 0.1778 3.7536 ± 0.0798 25.1566 ± 0.2723A 68.1127 ± 0.2102 30.3983 ± 0.1887 2.2768 ± 0.0275 27.7040 ± 0.1642
TT(22–24) 0.2129 ± 0.0064Bb 14.2950 ± 0.2501 18.9209 ± 0.3330 56.8847 ± 0.7389 0.1642 ± 0.0045ab 6.0245 ± 0.3750 3.5526 ± 0.1650 26.8471 ± 0.5501B 67.8471 ± 0.4476 30.4643 ± 0.4116 2.2507 ± 0.0598 27.7666 ± 0.3516
P 0.0010** 0.4300 0.9118 0.4383 0.0190 0.6321 0.1072 0.0021* 0.7890 0.9791 0.9099 0.9710
 g.6903810G > A AA(16–21) 0.1852 ± 0.0068 14.2381 ± 0.2711 19.4313 ± 0.3637 57.8248 ± 0.8116 0.1630 ± 0.0053AB 5.8672 ± 0.3861 3.5731 ± 0.1706 25.0782 ± 0.5779 67.6381 ± 0.4921 30.6643 ± 0.4476 2.1938 ± 0.0634 28.8024 ± 0.3790Aa
AG(154–191) 0.1907 ± 0.0030 14.2502 ± 0.1111 18.9316 ± 0.1477 56.9864 ± 0.3386 0.1751 ± 0.0021A 6.1871 ± 0.1691 3.6361 ± 0.0768 24.9321 ± 0.2611 68.1679 ± 0.2033 30.2696 ± 0.1845 2.3059 ± 0.0266 27.4558 ± 0.1589Bb
GG(622–743) 0.1907 ± 0.0023 14.0972 ± 0.0800 19.1316 ± 0.1076 57.5775 ± 0.2508 0.1692 ± 0.0016B 6.1143 ± 0.1313 3.6545 ± 0.0602 24.8228 ± 0.2058 67.8738 ± 0.1482 30.4623 ± 0.1352 2.2670 ± 0.0196 27.8031 ± 0.1169ABc
P 0.6965 0.3037 0.1962 0.1177 0.0027* 0.6719 0.8620 0.8107 0.2087 0.4195 0.1027 0.0006**
 g.6903365C > A AA(86–107) 0.1840 ± 0.0041 13.8722 ± 0.1550 19.4709 ± 0.2047a 57.9027 ± 0.4677 0.1597 ± 0.0031A 6.0759 ± 0.2298 3.4734 ± 0.1041 24.7990 ± 0.3588 67.7737 ± 0.2806 30.7073 ± 0.2542a 2.2474 ± 0.0368 28.0306 ± 0.2207a
CC(322–383) 0.1898 ± 0.0028 14.0896 ± 0.1003 18.8996 ± 0.1348b 57.0679 ± 0.3097 0.1652 ± 0.0020AB 6.2036 ± 0.1599 3.6362 ± 0.0727 25.0637 ± 0.2475 68.2830 ± 0.1841 30.0458 ± 0.1674b 2.3118 ± 0.0244 27.4913 ± 0.1448b
CA(44–55) 0.1908 ± 0.0049 14.0436 ± 0.1845 19.2701 ± 0.2468ab 57.5534 ± 0.5502 0.1736 ± 0.0037B 6.2137 ± 0.2664 3.5773 ± 0.1236 25.4468 ± 0.4221 67.8333 ± 0.3333 30.3655 ± 0.3056ab 2.2236 ± 0.0451 28.0080 ± 0.2610ab
P 0.2709 0.3586 0.0103 0.1432 0.0032* 0.8193 0.2198 0.3687 0.0975 0.0221 0.0476 0.0114
 g.6902878 T > G GG(162–202) 0.1990 ± 0.0034a 14.1712 ± 0.1255 19.2493 ± 0.1668 57.8214 ± 0.3823 0.1723 ± 0.0025 6.2335 ± 0.1958 3.7811 ± 0.0867 25.7488 ± 0.2926 67.8664 ± 0.2288 30.4505 ± 0.2085 2.2678 ± 0.0303 27.9791 ± 0.1799
GT(1–2) 0.1396 ± 0.0210b 15.1152 ± 0.8492 17.5963 ± 1.1241 55.1627 ± 2.4664 0.2174 ± 0.0205 4.6700 ± 1.1690 3.2808 ± 0.5268 22.3692 ± 1.7890 70.2243 ± 1.4970 27.7265 ± 1.3769 2.6772 ± 0.1992 25.8210 ± 1.1776
TT(298–334) 0.1937 ± 0.0033a 13.9941 ± 0.1205 19.0195 ± 0.1603 57.8556 ± 0.3675 0.1697 ± 0.0024 6.0736 ± 0.1867 3.6985 ± 0.0842 25.3001 ± 0.2887 68.1192 ± 0.2203 30.2583 ± 0.1994 2.2932 ± 0.0291 27.8464 ± 0.1728
P 0.0035* 0.1368 0.1045 0.5484 0.0342 0.2492 0.3232 0.0318 0.1353 0.0844 0.0802 0.1353
 g.6901713 T > G GG(23–27) 0.1820 ± 0.0064AB 14.2657 ± 0.2465 19.3224 ± 0.3331 57.4973 ± 0.7218 0.1618 ± 0.0046 5.3282 ± 0.3472a 3.6417 ± 0.1598 24.7738 ± 0.5396AB 67.7758 ± 0.4361 30.6385 ± 0.4050 2.2379 ± 0.0579 28.0440 ± 0.3424
GT(241–289) 0.1866 ± 0.0027A 14.0054 ± 0.0977 18.9266 ± 0.1306 57.1601 ± 0.3031 0.1707 ± 0.0019 6.0394 ± 0.1533ab 3.6135 ± 0.0702 24.4844 ± 0.2400A 68.2066 ± 0.1805 30.1259 ± 0.1638 2.2896 ± 0.0240 27.4588 ± 0.1422
TT(524–639) 0.1960 ± 0.0024B 14.1040 ± 0.0818 19.1405 ± 0.1105 57.1691 ± 0.2570 0.1715 ± 0.0016 6.2935 ± 0.1335b 3.6568 ± 0.0609 25.1479 ± 0.2074B 68.0575 ± 0.1524 30.3322 ± 0.1387 2.2695 ± 0.0201 27.7208 ± 0.1193
P <.0001** 0.3793 0.1310 0.8921 0.0925 0.0034* 0.7300 0.0019* 0.4593 0.2162 0.4936 0.0446
  1. Note: LSM least square mean, SE standard error. P indicates the significances of the association analysis between each SNP and milk fatty acid traits. P is the raw value. *: P < 0.0083 (the significant association analysis after multiple testing, 0.05/N). **: P < 0.0017 (the significant association analysis after multiple testing, 0.01/N). N is the number of SNPs. Different letter (small letters: P < 0.05; capital letters: P < 0.01) superscripts indicate significant differences among the genotypes. The number in the brackets represents the number of cows for the corresponding genotype
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