Fig. 4

Molecular Phylogenetic analysis by Maximum Likelihood method. Tree reconstruction of evolutionary distances among putative sequences of the Bm86 gene of Mexican tick populations was inferred by using the Maximum Likelihood method based on the Kimura 2-parameter model. A discrete Gamma distribution was used to model evolutionary rate differences among sites (2 categories (+G, parameter = 0.0500)). Bootstrap evaluation adjusted for 1000 permutation repetitions. The main clades considered here are shown in brackets marked as A and B. Clade B has 5 subclades labeled from B1 to B5. A total of 165 complete sequences, considering amino acid residues from 20 to 627 from the Bm86 were used in the analysis, including 125 Mexican sequences (localities are marked with circles of different colors) and GenBank sequences: 14 from Texas (USA, orange triangles); 18 from Thailand (brown triangles); 1 from Australia (blue triangle); 1 from Argentina (light blue triangle); 1 from India (pink triangle); 1 from Brazil (yellow triangle); the only previous Mexican (green triangle) and 2 Mozambique sequences used as outgroup (black triangles)