Fig. 4

Differential expression ratios of Luc reporters carrying WT or MT miR-2899 with different doses of Spi-1 proto-oncogene (SPI1) gene. A luciferase reporter vector containing the SPI1 mRNA 3′-UTR co-transfected with ß-gal in MAC-T. WT, g. 42,198,087 G > A-GG; MT, g. 42,198,087 G > A-AA. MiR-2899 (CCS-bta-223-MR04) at quality of 100 ng to 300 ng dose dependently repressed the reporter activities. Mock-transfected cells treated with the pMIR-REPORT vector alone. CmiR001-MR04 served as a scrambled control. Data are representative of four separate experiments (mean ± SD)