Table 2 List of primers used to follow the mutation of BoFLC1.C9 gene in parental lines, F1 and segregating F2 by polymerase chain reaction (PCR); and relative expression analysis of the target and Actin genes in quantitative PCR (qPCR)
Gene name | Accession number | Primer code | Sequences (5′→3′) | Product size (bp) | Marker type | References |
|---|---|---|---|---|---|---|
BoFLC1.C9 | Bo9g173400 | F7: R7: | GGAAAGCAACATGGTGATGA CATGGTGTGAACCAGAGTCC | 438 | Indel | Present study |
F: R: | CTCTACAGCTTCTCCTCCGG TGTGAACCAGAGTCCAAAGC | 119 | qPCR | Present study | ||
Actin | AF044573 | 1F: 1R: | TTCTCTCTTCCACACGCCAT CTTGTCCTGCGGGTAATTCG | 235 | [62] | |
JQ435879 | 2F: 2R: | GTCGCTATTCAAGCTGTTCTCT GAGAGCTTCTCCTTGATGTCTC | 251 | [63] | ||
XM_013753106 | 3F: 3R: | ATCACACTTTCTACAATGAGC TCGTAGATTGGCACAGTGTGAG | 241 | [64] |