Skip to main content
Fig. 3 | BMC Genetics

Fig. 3

From: A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism

Fig. 3

dCNDP2 is present in both the cytoplasm and the nucleus. a Western blot showing subcellular fractionation of S2 cells. b Western blot showing ectopic expression of eGFP-tagged proteins in salivary glands using the AB1-GAL4 driver. L3, third instar larval stage. c Localization of eGFP-tagged proteins in L3 salivary gland cells. GFP.nls and mCD8:GFP were used as controls for nuclear localization, and cytoplasmic and transmembrane localization, respectively. Scale bar, 20 μm. d dCNDP2 binds multiple sites on polytene chromosomes. The following exposure times were used to acquire images of chromosomes immunostained with α-dCNDP2 antibodies: 800 msec, ∆dCNDP2 and ∆dCNDP2(rescue); 500 msec, AB1-GAL4 > UAS-dCNDP2. Scale bar, 20 μm

Back to article page