Fig. 2From: The transcription factor PRO44 and the histone chaperone ASF1 regulate distinct aspects of multicellular development in the filamentous fungus Sordaria macrosporaPRO44 forms homodimers. a Multiple alignment of the genomic region around the second intron in pro44. The three splice variants of pro44 (pro44-A, -B, and -C) are shown. b Yeast-two-hybrid analysis of interactions of different PRO44 variants resulting from different splice variants. c Co-immunoprecipitation of two differently tagged PRO44 proteins (EGFP- and TAP-tagged proteins in strain S135826, only EGFP-PRO44 in strain SFA198, only TAP-PRO44 in strain SFA4019). The band seen in strain SFA198 (EGFP-PRO44) after TAP-IP and western blot with an anti-EGFP antibody is unspecific (for details see Additional file 2: Figure S2)Back to article page