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Table 5 Validation of the two quantitative trait loci (QTL) in structured and unstructured recombinant inbred line (RIL) populations with the corresponding senescence effect

From: Identification and validation of QTL and their associated genes for pre-emergent metribuzin tolerance in hexaploid wheat (Triticum aestivum L.)

Co-located QTL for CCI and SNS

RIL population

Marker

AA

aa

P valueb

Effect (%)c

Qcci.uwa.1AS and Qsns.uwa.1AS

Synthetic W7984 × Westonia

Xgwm33

4.0a

5.6

0.03*

28.5

Synthetic W7984 × Lang

Xgwm33

4.2a

5.9

0.01**

28.8

Qcci.uwa.4AL.1 and Qsns.uwa.4AL.1

Synthetic W7984 × Westonia

Xbarc343

4

5.3

0.04*

24.5

Synthetic W7984 × Lang

Xbarc343

4.7

5.6

0.02*

9.6

  1. AA homozygous alleles from Synthetic W7984, aa homozygous alleles from Westonia and Lang
  2. aPhenotypic effect for null bands
  3. bStudent’s t-test (P < 0.05) was used to identify differences between the lines of population with distinct allele peaks; **, significant at P < 0.01; *, significant at P < 0.05
  4. cAverage decrease in senescence