Table 2 Summary of results obtained after hybridization of BACs by two- and multi-color FISH technique and annotation of BAC-contigs and a BAC-unitig
Chrom. | Probe | BAC- contig | Genes |
|---|---|---|---|
1 | -- | – | -- |
2 | BAC Ba 102 L1 | Contig 9 | NF-kappa-B inhibitor cactus; DNA replication complex GINS protein PSF1; Adenylosuccinate synthetase; Mitochondrial import inner membrane translocase subunit Tim9; Novel protein coding; Enhanced at puberty protein 1-like protein B; Microtubule-associated proteins 1A/1B light chain 3C. |
Ba177C21 | Contig 10 | Novel protein coding; ADAMTS-like protein 3; F-box only protein 22; Novel protein coding; Novel protein coding; | |
Ba50F9 | Contig 11 | MT-IIG (C. virginica), Filamin-A-like (C.gigas predicted) | |
3 | H4 | -- | H4 histone |
BAC Ba17L12 | Contig 5 | Ribonuclease P protein subunit p40; Kelch-like protein 13; Sodium-and chloride-dependent betaine transporter; Pleckstrin-like protein domain-containing family B member 2; Proteasome subunit beta type-8; Novel protein coding; Novel protein coding; Steroid 17-alpha-hydroxylase/17,20 lyase; E3 ubiquitin-protein ligase MYLIP; Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial | |
BAC Ba18I16 | Contig 8 | Kelch-like protein 13; Sodium-and chloride-dependent betaine transporter; Pleckstrin-like protein domain-containing family B member 2; Proteasome subunit beta type-8; Novel protein coding; Novel protein coding; Novel protein coding; Steroid 17-alpha-hydroxylase/17,20 lyase; E3 ubiquitin-protein ligase MYLIP; Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial; | |
4 | 5S rDNA | -- | 5S Ribosomal DNA |
BAC Ba106O6 | Contig 1 | Metallothionein; Novel protein coding; Filamin-C; Novel protein coding; Novel protein coding; Nudix hydrolase 20; JmjC domain-containing protein C2orf60-like protein; Novel protein coding; Novel protein coding; Tripartite motif-containing protein 3; Neurogenic locus notch-like protein 4; Caveolin; Metallothionein; Metallothionein; | |
5 | 5S rDNA | -- | 5S ribosomal DNA |
BAC Ba3D14 | Contig 2 | Myeloid differentiation primary response protein MyD88; Novel protein coding; Myeloid differentiation primary response protein MyD88; Novel protein coding; Myeloid differentiation primary response protein MyD88; Putative GTP-binding protein 6; LIM domain and actin-binding protein 1; Novel protein coding; Novel protein coding; Calcineurin-binding protein cabin-1; Di-N-acetylchitobiase. | |
6 | Ba127F5 | Unitig-Ba127F5 | Serine/threonine-protein kinase 19; Protein Dom3Z; ZZ-type zinc finger-containing protein 3; Rhodopsin, GQ-coupled; C3a anaphylatoxin chemotactic receptor; Melatonin receptor type 1B; Melatonin receptor type 1B; G-protein coupled receptor moody. |
7 | BAC Ba133H22 | Contig 3 | Thyroid transcription factor 1-associated protein 26; Orexin receptor type 2; Novel protein coding; WD repeat-containing protein 86; WD repeat-containing protein 86; Novel protein coding; Novel protein coding; Cys-loop ligand-gated ionic channel. |
BAC Ba96P24 | Contig 6 | Galanin receptor type 2; Alpha-2A adrenergic receptor; Thyroid transcription factor 1-associated protein 26; Orexin receptor type 2; Novel protein coding; Novel protein coding; Cys-loop ligand-gated ionic channel. | |
8 | BAC Ba13P13 | Contig 4 | Novel protein coding; Tripartite motif-containing protein 45; Complement C1q-like protein 3; Tripartite motif-containing protein 45; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; Novel protein coding; Neurogenic locus notch-like protein 2; Putative imidazolonepropionase; Coiled-coil domain-containing protein 37; Ribonuclease P protein subunit p30; Novel protein coding; Tryptase gamma; Protein sidekick-2; Tyrosine-protein phosphatase 99A; Metalloproteinase inhibitor 1; Tissue inhibitor of metalloproteinase TIMP; Metalloproteinase inhibitor 3; Tissue inhibitor of metalloproteinase TIMP 1.1; WD40 repeat-containing protein SMU1; N-acetyltransferase ats1; Novel protein coding. |
BAC Ba45B21 | Contig 7 | Myeloid differentiation primary response protein MyD88; Novel protein coding; Heavy metal-binding protein HIP; Myeloid differentiation primary response protein MyD88; Novel protein coding; Myeloid differentiation primary response protein MyD88; Thioredoxin-like protein 4A; Putative GTP-binding protein 6; LIM domain and actin-binding protein 1; Novel protein coding; Novel protein coding; Disrupted in renal carcinoma protein 2-like protein | |
9 | -- | -- | -- |
10 | 18S rDNA | -- | 18S ribosomal DNA |